– Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure.  CF® dyes are Biotium’s line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Far-red/near-IR fluorescent CF®680R has excitation/emission at 680/701 nm.  Kit Components: 

 
• CF®680R tyramide stock solution
 
• HRP Goat anti-mouse IgG
 
• BSA for blocking
 
• Amplification buffer
 
• Hydrogen peroxide 30% stablized solution
 

  We also offer Tyramide Amplification Kits with HRP goat anti-rabbit or HRP streptavidin.

Source / Ingredients

IgG from domesticated goat serum (Capra aegagrus hircus); BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells; peroxidase from Horseradish (Armoracia rusticana)

Handling

Protect from light;