– EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making it readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen® dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. EvaGreen® dye is nonmutagenic and noncytotoxic because it is impermeable to cell membranes, unlike SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer.  The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.  See our ready-to-use Forget-Me-Not™ EvaGreen® qPCR master mixes for sensitive, competitively-priced qPCR reagents. We also offer Thiazole Green, 1000X in DMSO, which is identical to SYBR® Green I.  EvaGreen dye and applications are covered by granted US and international patents.  

Features:

  Low PCR inhibition Exhibits much less PCR inhibition than SYBR® Green I via a smart “release-on-demand” DNA-binding technology  Highly Sensitive Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals  Nonmutagenic and noncytotoxic Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes  Compatible with fast PCR protocols Minimal interference to PCR makes it possible to significantly shorten the chain extension time  Compatible with multiplex PCR No dye migration from amplicon to amplicon when used at the recommended concentration  Unsurpassed thermal stability, chemical stability and photostability No detectable dye decomposition in PCR buffer at 95-100°C for 48 hours; highly stable under either alkaline or acidic condition; withstands repeated freeze-thaw cycles  Spectrally similar to SYBR® Green I Compatible with all major brand qPCR instruments and enzyme systems

Handling

Protect from light;