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10-Acetyl-3, 7-dihydroxyphenoxazine
SKU
BT-10061
Categories Fluorescence Microscopy, Other Enzyme Substrates, Super-Resolution Imaging
Brand: Biotium
301 CAD
Only 1000 item(s) left in stock.
For Research Use Only (RUO). This product is sold strictly for laboratory research use. Not for human or veterinary consumption, therapeutic, or diagnostic use.For sale to research organizations and laboratories only; not for patients or for personal use.
Products Details
Product Description
– 10-Acetyl-3,7-dihydroxyphenoxazine (N-Acetyl-3,7-dihydroxyphenoxazine or ADHP), which has been marketed under the name Amplex® Red, is regarded as the best fluorogenic substrate for peroxidase because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H2O2(reacting in a 1:1 stoichiometry) in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. H2O2 is produced by many enzymatic reactions, and thereforeADHP can be used to detect the activity of many different enzymes.
- Detect HRP in immunoassays
- Detect trace amounts of hydrogen peroxide (H2O2)
- Detect using fluorometer or microplate reader
- λEx/λEm(pH 9)= 571/585 nm (end product)
- ε= 54,000 (end product)
- White to off-white solid soluble in DMSO or DMF
- Store desiccated at -20°C and protect from light
- C14H11CNO4
- MW: 257.24
- [119171-73-2]
Handling
Store with desiccant; Protect from light
For Research Use Only (RUO). This product is sold strictly for laboratory research use only. Not for human or veterinary consumption, therapeutic, or diagnostic use. Any pharmacological, therapeutic, [cosmetic chemistry research literature], or biological effects described above are provided as research context only and do not represent intended or approved uses of this product.
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
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