– RedDot™1 is a far-red cell membrane-permeable nuclear dye similar to Draq5™. The dye is ideal for specifically staining the nuclei of live cells for short term imaging. RedDot™1 can be used to stain adherent or suspension cells. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. RedDot™1 has excitation/emission maxima at 662/694 nm, for detection in the Cy®5 channel. It also can be excited at a wide range of wavelengths between 488 nm and 647 nm, including the 488 nm laser for flow cytometry, and can be used for DNA content analysis by flow cytometry. RedDot™1 also can be used for normalization of In Cell Western® in fixed cells, similar to Draq5™/Sapphire®700.  Please note: far-red dyes like RedDot™1 are not visible to the human eye, but must be imaged with a CCD camera or by confocal microscopy.  Like Draq5®, RedDot™1 is toxic to cells within a few hours, and is recommended for short term staining experiments. For a low-toxicity far-red nuclear stain for live cells, see our NucSpot™ 650 dye. Please also see RedDot™2, a spectrally similar dye designed for specific nuclear staining of fixed and permeabilized cells or selective nuclear staining of dead cells.  Cy dye is a registered trademark of GE Healthcare. Draq5 is a trademark of BioStatus Ltd. In Cell Western and Sapphire are registered trademarks of LI-COR Inc.

Applications

Live cell nuclear staining, In Cell Western normalization

Handling

Protect from light;