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PathoScreen® Kit for Xylella fastidiosa (Xf)
$286 – $799
Products Details
Product Description
– This ELISA product is a qualitative assay for the detection of Xylella fastidiosa (Xf), the causal agent of bacterial leaf scorch, Pierce’s disease, and phony peach disease. This pathogen has a wide host range, including almond, blackberry, citrus, grapevine, and olive.
The antibodies for this assay were screened against multiple isolates of three subspecies within the X. fastidiosa species, detecting all true positives, including those from infected Oleander. Exclusivity panels revealed no cross-reactivity with multiple common bacterial, fungal and viral pathogens. Moreover, no host-reaction was observed with healthy tissue from any common host of X. fastidiosa we tested.
Xylella fastidiosa spreads throughout the plant via the xylem vessels and can be distributed differentially within the host. Due to this localization, we recommend testing petiole or stem samples from multiple locations on symptomatic plants. Please note that continuing external research suggests root tissue provides the most consistent accurate results when testing peach for the presence of X. fastidiosa.
The antibodies for this assay were screened against multiple isolates of three subspecies within the X. fastidiosa species, detecting all true positives, including those from infected Oleander. Exclusivity panels revealed no cross-reactivity with multiple common bacterial, fungal and viral pathogens. Moreover, no host-reaction was observed with healthy tissue from any common host of X. fastidiosa we tested.
Xylella fastidiosa spreads throughout the plant via the xylem vessels and can be distributed differentially within the host. Due to this localization, we recommend testing petiole or stem samples from multiple locations on symptomatic plants. Please note that continuing external research suggests root tissue provides the most consistent accurate results when testing peach for the presence of X. fastidiosa.
Web ID
– PSA 34503
Shipping
– Room temperature
Kit Components
– Antibody-coated 96-well microtiter plates
Alkaline phosphatase enzyme conjugate
RUB6, 1X Ready to use
PNP substrate tablets, 5 mg each
PNP substrate buffer, 5X concentrate
Positive control
PBST buffer, 20X concentrate, 50 mL
TWEEN® 20
General extract buffer, GEB
User Guide
Alkaline phosphatase enzyme conjugate
RUB6, 1X Ready to use
PNP substrate tablets, 5 mg each
PNP substrate buffer, 5X concentrate
Positive control
PBST buffer, 20X concentrate, 50 mL
TWEEN® 20
General extract buffer, GEB
User Guide
Features & Benefits
– Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal
Limit of detection: 2×106 CFU/mL
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal
Limit of detection: 2×106 CFU/mL
Disclaimer: All products are for research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation.
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