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AmplifyRP® XRT for BCTV (Beet curly top virus)
1,200 CAD
Only 1000 item(s) left in stock.
Products Details
Product Description
– AmplifyRP® XRT for BCTV is a rapid DNA amplification and detection platform designed for field-based or laboratory testing of Beet curly top virus in many crops including: bean, beet, hemp (Cannabis spp.), pepper, potato, spinach, and tomato. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify BCTV DNA at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( – ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
The test can be performed virtually anywhere using the battery operated AmpliFire fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( – ).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( – ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
The test can be performed virtually anywhere using the battery operated AmpliFire fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( – ).
Web ID
– XCS 11000
Shipping
– Room temperature
Kit Components
– XRT reaction pellets for BCTV
Pre-filled 100 µL PD1 Pellet diluent tubes
GEB-filled mesh extraction bags
User Guide
Pre-filled 100 µL PD1 Pellet diluent tubes
GEB-filled mesh extraction bags
User Guide
Features & Benefits
– Test Format: AmplifyRP® XRT
Target Label: FAM-labeled target probe
Internal Control: N/A
Limit of detection: Approximately 1 fg DNA fragment; 1500 copies/µL
Isolate Names / Geographic Strain Locations Analytical Sensitivity
BCTV-CA (CA, USA) Unknown
BCTV-Can (USA) (Cannabis) Unknown
BCTV-CO (CO, USA) 1:10,000 dilution of infected tissue (pathogen titer unknown)
BCTV-KIM1 (ID, USA) (Kimberly1) Unknown
BCTV-LH71 (CA, USA) (Leafhopper 71) 1 fg / μL DNA fragment; 1800 copies
BCTV-Logan (CA, USA) Unknown
BCTV-Mld (Mexico) (Mild) 10 ag / μL DNA fragment; 20 copies
BCTV-PeCT (NM, USA) (Pepper Curly Top) 1:1,000 dilution of infected tissue (pathogen titer unknown)
BCTV-PeYD2 (NM, USA) (Pepper Yellow Dwarf) Unknown
BCTV-SpCT1 (USA) (Spinach Curly Top) 3 fg / μL DNA fragment; 5500 copies
BCTV-Svr (BCTV-CFH) (Severe) 1:1,000 dilution of infected tissue (pathogen titer unknown)
BCTV-SvrPep (NM, USA) (Severe pepper) 10 ag / μL DNA fragment; 20 copies
BCTV-Wor (Worland) Unknown
Target Label: FAM-labeled target probe
Internal Control: N/A
Limit of detection: Approximately 1 fg DNA fragment; 1500 copies/µL
Isolate Names / Geographic Strain Locations Analytical Sensitivity
BCTV-CA (CA, USA) Unknown
BCTV-Can (USA) (Cannabis) Unknown
BCTV-CO (CO, USA) 1:10,000 dilution of infected tissue (pathogen titer unknown)
BCTV-KIM1 (ID, USA) (Kimberly1) Unknown
BCTV-LH71 (CA, USA) (Leafhopper 71) 1 fg / μL DNA fragment; 1800 copies
BCTV-Logan (CA, USA) Unknown
BCTV-Mld (Mexico) (Mild) 10 ag / μL DNA fragment; 20 copies
BCTV-PeCT (NM, USA) (Pepper Curly Top) 1:1,000 dilution of infected tissue (pathogen titer unknown)
BCTV-PeYD2 (NM, USA) (Pepper Yellow Dwarf) Unknown
BCTV-SpCT1 (USA) (Spinach Curly Top) 3 fg / μL DNA fragment; 5500 copies
BCTV-Svr (BCTV-CFH) (Severe) 1:1,000 dilution of infected tissue (pathogen titer unknown)
BCTV-SvrPep (NM, USA) (Severe pepper) 10 ag / μL DNA fragment; 20 copies
BCTV-Wor (Worland) Unknown
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
