– BODIPY TR methyl ester serves as a lipophilic GFP counterstain, easily permeating cell membranes to accumulate in endomembranous organelles, while exhibiting minimal localization in plasma membranes. It is a highly effective red fluorescent vital dye (Ex=568 nm, Em=625 nm) that can reveal the location and shape of cell nuclei, the morphology of cells in embryonic tissues, and the boundaries of organ-forming tissues within an entire embryo [1].