– 2′-Hydroxychalcone is a drug synthesis intermediate for the synthesis of flavonoids. 2′-Hydroxychalcone loaded in nanoemulsion showed fungicidal activity against Coccidioides parapsilosis in Danio rerio model.2′-Hydroxychalcone induced cytotoxicity through oxidative stress in lipid-loaded Hepg2 cells.2′-Hydroxychalcone inhibited the induction of ICAM-1, VCAM-α, tumor necrosis factor-α, and tumor necrosis factor-alpha as determined by reverse transcription-polymerase chain reaction determined by tumor necrosis factor-alpha induces steady-state transcript levels of ICAM-1, VCAM-1, and E-selectin, and therefore may interfere with the transcription of their genes.