– Universal SYBR Green qPCR Master Mix is a ready-to-use 2× concentrated premix formulated for real-time quantitative PCR (qPCR). It contains a hot-start DNA polymerase, SYBR Green I fluorescent dye, dNTPs, Mg²⁺, and an optimized ROX reference dye, making it compatible with all qPCR instruments. To perform a reaction, simply add the DNA template, primers, and nuclease-free water—streamlining the workflow and minimizing the risk of contamination. This Master Mix incorporates a temperature-responsive polymerase activator that precisely regulates enzyme activity in real time. The formulation also includes proprietary components that effectively suppress non-specific amplification and enhance overall reaction efficiency. As a result, it enables accurate and reproducible quantification of target genes across a broad dynamic range, ensuring high sensitivity, specificity, and reliability in qPCR applications.