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Rat VD3(Vitamin D3) ELISA Kit
$212 – $301
Products Details
Product Description
– This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat VD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Web ID
– IM0598KLE
Applications
– Competitive Inhibition ELISA
Research Area
– Metabolic pathway,Reproductive science,Nutrition metabolism,Bone metabolism,
Features & Benefits
– Competitive Inhibition ELISA with sensitivity of 2.14 ng/mLand detection range of 6.25-400 ng/mL. Kit includes a standard of 400 ng/mL and takes a total assay time of 2h. This kit can be used to assay serum, plasma and other biological fluids.
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
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