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Rat VCAM1(Vascular Cell Adhesion Molecule 1) ELISA Kit
SKU
IM7952KLE-96T
Category Rat RUO ELISA
Tags CD & Adhesion molecule, Infection immunity, Tumor immunity
$256 – $366
Products Details
Product Description
– The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VCAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VCAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VCAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VCAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Web ID
– IM7952KLE
Applications
– Sandwich ELISA
References
– UnitProt ID:P29534
Research Area
– CD & Adhesion molecule,Tumor immunity,Infection immunity,
Features & Benefits
– Sandwich ELISA with sensitivity of 0.45 ng/mLand detection range of 1.25-80 ng/mL. Kit includes a standard of 80 ng/mL and takes a total assay time of 3.5h. This kit can be used to assay Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Disclaimer: All products are for research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation.
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