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Rat TRH(Thyrotropin Releasing Hormone) ELISA Kit
$256 – $366
Products Details
Product Description
– This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRH in the samples is then determined by comparing the OD of the samples to the standard curve.
Web ID
– IM7062KLE
Applications
– Competitive Inhibition ELISA
References
– UnitProt ID:P01150
Research Area
– Endocrinology,Neuro science,Hepatology,Hormone metabolism,
Features & Benefits
– Competitive Inhibition ELISA with sensitivity of 2.43 pg/mLand detection range of 7.82-500 pg/mL. Kit includes a standard of 500 pg/mL and takes a total assay time of 2h. This kit can be used to assay serum, plasma and other biological fluids.
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
