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Rabbit S100A11(S100 Calcium Binding Protein A11) ELISA Kit
SKU
IM5655KLE-96T
Category Rabbit RUO ELISA
Tags Developmental science, Infection immunity, Tumor immunity
$330 – $470
Products Details
Product Description
– The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit S100A11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit S100A11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit S100A11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit S100A11 in the samples is then determined by comparing the OD of the samples to the standard curve.
Web ID
– IM5655KLE
Applications
– Sandwich ELISA
References
– UnitProt ID:P24480
Research Area
– Tumor immunity,Infection immunity,Developmental science,
Features & Benefits
– Sandwich ELISA with sensitivity of 0.061 ng/mLand detection range of 0.16-10 ng/mL. Kit includes a standard of 10 ng/mL and takes a total assay time of 3.5h. This kit can be used to assay serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
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