Mouse TUBb1(Tubulin Beta 1) ELISA Kit

SKU IM4236KLE-48T Category Tag

$256$366

Products Details

Product Description

– The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TUBb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TUBb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TUBb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TUBb1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Web ID

– IM4236KLE

Applications

– Sandwich ELISA

References

– UnitProt ID:A2AQ07

Research Area

– Infection immunity,

Features & Benefits

– Sandwich ELISA with sensitivity of 0.063 ng/mLand detection range of 0.16-10 ng/mL. Kit includes a standard of 10 ng/mL and takes a total assay time of 3.5h. This kit can be used to assay Tissue homogenates, cell lysates and other biological fluids..

Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.

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