Mouse APLN(Apelin) ELISA Kit

SKU IM2107KLE-48T Category Tag

$256$366

Products Details

Product Description

– This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse APLN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APLN in the samples is then determined by comparing the OD of the samples to the standard curve.

Web ID

– IM2107KLE

Applications

– Competitive Inhibition ELISA

References

– UnitProt ID:Q9R0R4

Research Area

– Endocrinology,

Features & Benefits

– Competitive Inhibition ELISA with sensitivity of 35.3 pg/mLand detection range of 125-8000 pg/mL. Kit includes a standard of 8000 pg/mL and takes a total assay time of 2h. This kit can be used to assay Serum, plasma and other biological fluids..

Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.

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