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Human ABL1(Tyrosine-protein kinase ABL1) ELISA Kit
$256 – $366
Products Details
Product Description
– The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ABL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ABL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ABL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ABL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Web ID
– IM8289KLE
Applications
– Sandwich ELISA
References
– UnitProt ID:P00519
Research Area
– Signal Transduction, Cancer, Epigenetics and Nuclear Signaling, Neuroscience
Features & Benefits
– Sandwich ELISA with sensitivity of 16.4 pg/mLand detection range of 62.5-4000 pg/mL. Kit includes a standard of 4000 pg/mL and takes a total assay time of 3.5h. This kit can be used to assay serum, plasma, tissue homogenates.
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
