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Horse 5-HT(5-Hydroxytryptamine) ELISA Kit

$212$301

Products Details

Product Description

– This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Horse 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.

Web ID

– IM4830KLE

Applications

– Competitive Inhibition ELISA

Research Area

– Signal transduction,Infection immunity,Neuro science,Gastroenterology,

Features & Benefits

– Competitive Inhibition ELISA with sensitivity of 6.12 ng/mLand detection range of 14.07-900 ng/mL. Kit includes a standard of 900 ng/mL and takes a total assay time of 2.5h. This kit can be used to assay serum, plasma, tissue homogenates.

Disclaimer: All products are for research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation.

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