Download Files:
H4(Histone H4) ELISA Kit
$212 – $301
Products Details
Product Description
– The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human,Rat,Mouse H4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human,Rat,Mouse H4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human,Rat,Mouse H4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human,Rat,Mouse H4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Web ID
– IM2584KLE
Applications
– Sandwich ELISA
References
– UnitProt ID:P62805
Research Area
– Signal transduction,Developmental science,
Features & Benefits
– Sandwich ELISA with sensitivity of 0.056 ng/mLand detection range of 0.16-10 ng/mL. Kit includes a standard of 10 ng/mL and takes a total assay time of 3.5h. This kit can be used to assay tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
