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EasyStep Mouse 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit
SKU
IMSE250KLE-96T
Category Mouse RUO ELISA
Tags Bone metabolism, Genetic science, Nutrition metabolism, Reproductive science
$330 – $470
Products Details
Product Description
– This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 25-OH-D antigen, and the Mouse 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.
Web ID
– IMSE250KLE
Applications
– Competitive Inhibition ELISA
Research Area
– Reproductive science,Genetic science,Nutrition metabolism,Bone metabolism,
Features & Benefits
– Competitive Inhibition ELISA with sensitivity of 7.7 ng/mLand detection range of 78.13-5000 ng/mL. Kit includes a standard of 5000 ng/mL and takes a total assay time of 1.5h. This kit can be used to assay Serum, plasma.
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
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