EasyStep Human NLRP3(NLR Family, Pyrin Domain Containing Protein 3) ELISA Kit

SKU IMSE930KLE-96T Category Tag

$330$470

Products Details

Product Description

– The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NLRP3, and the Human NLRP3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NLRP3. After TMB substrate solution is added, only those wells that contain Human NLRP3 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NLRP3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Web ID

– IMSE930KLE

Applications

– Sandwich ELISA

References

– UnitProt ID:D4A523

Research Area

– Signal transduction,

Features & Benefits

– Sandwich ELISA with sensitivity of 37 pg/mLand detection range of 0.16-10 ng/mL. Kit includes a standard of 10 ng/mL and takes a total assay time of 1.5h. This kit can be used to assay Serum, plasma.

Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.

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