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Dog C3a(Complement Component 3a) ELISA Kit
$330 – $470
Products Details
Product Description
– The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog C3a in the samples is then determined by comparing the OD of the samples to the standard curve.
Web ID
– IM0306KLE
Applications
– Sandwich ELISA
Research Area
– Infection immunity,Immune molecule,Autoimmunity,
Features & Benefits
– Sandwich ELISA with sensitivity of 1.29 ng/mLand detection range of 3.13-200 ng/mL. Kit includes a standard of 200 ng/mL and takes a total assay time of 3.5h. This kit can be used to assay serum and plasma.
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
