Download Files:
Products Details
Product Description
– AmplifyRP® XRT for GLRaV-3 is a rapid RNA amplification and detection platform designed for testing grapevines for Grapevine leafroll-associated virus 3. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify GLRaV-3 RNA and an endogenous RNA control at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
XCS 51300/0048
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
XCS 51300/0048
Web ID
– XCS 29001
Shipping
– Room temperature
Kit Components
– XRT reaction pellets for GLRaV-3 (48)
PD1 Pellet diluent (55 mL)
1.5 mL microcentrifuge tubes, graduated (50)
GEB-filled mesh extraction bags (50)
User Guide
PD1 Pellet diluent (55 mL)
1.5 mL microcentrifuge tubes, graduated (50)
GEB-filled mesh extraction bags (50)
User Guide
Features & Benefits
– Test Label: FAM-labeled target probe
Test Format: AmplifyRP® XRT
Internal Control: Yes – Endogenous
Limit of Detection (LOD): 2.5 fg RNA transcript per reaction
Specificity:
Inclusive: Designed to detect all GLRaV-3 isolates.
Exclusive: Does not detect: Arabis mosaic virus (ArMV), Botrytis cinerea, Grapevine fanleaf virus (GFLV), Grapevine fleck virus (GFkV), Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine leafroll-associated virus 2 (GLRaV-2), Grapevine leafroll-associated virus 4 (GLRaV-4), Grapevine leafroll-associated virus 7 (GLRaV-7), Grapevine leafroll-associated virus 10 (GLRaV-10), Grapevine pinot gris virus (GPGV), Grapevine red blotch virus (GRBV), Little cherry virus 2 (LChV2), Phytoplasma solani, Phytoplasma vitis, Tobacco ringspot virus (TRSV), Tomato ringspot virus (ToRSV), and Xylella fastidiosa (Xf)
Predicted not to detect via insilico data: Grapevine leafroll-associated virus 5 (GLRaV-5), Grapevine leafroll-associated virus 6 (GLRaV-6), Grapevine leafroll-associated virus 9 (GLRaV-9), Grapevine leafroll-associated virus Car (GLRaV-Car), Grapevine leafroll-associated virus De (GLRaV-De), Grapevine leafroll-associated virus Pr (GLRaV-Pr), Peach rosette mosaic virus (PRMV), Pineapple mealybug wilt associated virus 1 (PMWaV-1), Pineapple mealybug wilt associated virus 2 (PMWaV-2), Pineapple mealybug wilt associated virus 3 (PMWaV-3), Plum bark necrosis stem pitting-associated virus (PBNSPaV), and Strawberry latent ringspot virus (SLRSV)
Test Format: AmplifyRP® XRT
Internal Control: Yes – Endogenous
Limit of Detection (LOD): 2.5 fg RNA transcript per reaction
Specificity:
Inclusive: Designed to detect all GLRaV-3 isolates.
Exclusive: Does not detect: Arabis mosaic virus (ArMV), Botrytis cinerea, Grapevine fanleaf virus (GFLV), Grapevine fleck virus (GFkV), Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine leafroll-associated virus 2 (GLRaV-2), Grapevine leafroll-associated virus 4 (GLRaV-4), Grapevine leafroll-associated virus 7 (GLRaV-7), Grapevine leafroll-associated virus 10 (GLRaV-10), Grapevine pinot gris virus (GPGV), Grapevine red blotch virus (GRBV), Little cherry virus 2 (LChV2), Phytoplasma solani, Phytoplasma vitis, Tobacco ringspot virus (TRSV), Tomato ringspot virus (ToRSV), and Xylella fastidiosa (Xf)
Predicted not to detect via insilico data: Grapevine leafroll-associated virus 5 (GLRaV-5), Grapevine leafroll-associated virus 6 (GLRaV-6), Grapevine leafroll-associated virus 9 (GLRaV-9), Grapevine leafroll-associated virus Car (GLRaV-Car), Grapevine leafroll-associated virus De (GLRaV-De), Grapevine leafroll-associated virus Pr (GLRaV-Pr), Peach rosette mosaic virus (PRMV), Pineapple mealybug wilt associated virus 1 (PMWaV-1), Pineapple mealybug wilt associated virus 2 (PMWaV-2), Pineapple mealybug wilt associated virus 3 (PMWaV-3), Plum bark necrosis stem pitting-associated virus (PBNSPaV), and Strawberry latent ringspot virus (SLRSV)
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
Related Products
1000 in stock