– 4-(2,4-Dinitroanilino)phenol serves as a multifunctional dye, essential in both biological experimentation and various industries. In biology, dyes enable researchers to observe cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology, and monitor microorganisms. Their uses extend from fundamental scientific research to clinical diagnostics. Additionally, dyes are prevalent in traditional sectors like textile dyeing and are gaining importance in emerging fields such as functional textile processing, food pigments, and dye-sensitized solar cells.