– α-Galactosidase (EC 3.2.1.22), a glycoside hydrolase enzyme, is prevalent across animals, plants, and microorganisms, and is often utilized in biochemical research. It facilitates the hydrolysis of α-1,6-linked terminal galactose residues in substrates such as galactooligosaccharides, galactomannans, and galactolipids. This includes catalyzing key catabolic processes involved in the cleavage of glycoproteins, glycolipids, and polysaccharides [1].