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Human HS(Heparan Sulfate) ELISA Kit

$212$301

Products Details

Product Description

– This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HS protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HS in the samples is then determined by comparing the OD of the samples to the standard curve.

Web ID

– IM8670KLE

Applications

– Competitive Inhibition ELISA

Research Area

– Signal transduction,Metabolic pathway,Tumor immunity,Infection immunity,Developmental science,

Features & Benefits

– Competitive Inhibition ELISA with sensitivity of 51.3 ng/mLand detection range of 125-8000 ng/mL. Kit includes a standard of 8000 ng/mL and takes a total assay time of 2h. This kit can be used to assay serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Disclaimer: All products are for research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation.

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