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Human HLA-B27(Leukocyte Antigen B27) ELISA Kit
$256 – $366
Products Details
Product Description
– The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HLA-B27. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HLA-B27. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HLA-B27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HLA-B27 in the samples is then determined by comparing the OD of the samples to the standard curve.
Web ID
– IM4680KLE
Applications
– Sandwich ELISA
References
– UnitProt ID:O19193
Research Area
– Immune molecule
Features & Benefits
– Sandwich ELISA with sensitivity of 0.12 ng/mLand detection range of 0.32-20 ng/mL. Kit includes a standard of 20 ng/mL and takes a total assay time of 3.5h. This kit can be used to assay serum, plasma, tissue homogenates and other biological fluids.
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
