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Products Details
Product Description
– This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.
Web ID
– IM5948KLE
Applications
– Competitive Inhibition ELISA
References
– UnitProt ID:P37042
Research Area
– Endocrinology,Reproductive science,Neuro science,Genetic science,Hormone metabolism,
Features & Benefits
– Competitive Inhibition ELISA with sensitivity of 0.1 ng/mLand detection range of 0.25-16 ng/mL. Kit includes a standard of 16 ng/mL and takes a total assay time of 2h. This kit can be used to assay serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
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