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Product Description
– Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays[1][2][3].
Web ID
– HY-D0952
Storage Temperature
– 4°C (Powder, protect from light)
Shipping
– Room Temperature
Molecular Formula
– C17H19N3
Citations
– Chemosphere. 2023 Apr;320:138071.|Research Square Print. December 12th, 2022.|Acta Pharm Sin B. 2021 Feb 11.|Adv Funct Mater. 2023 Apr 14.|Cell Death Dis. 2020 Jul 30;11(7):607.|Cell Death Dis. 2021 Jan 13;12(1):80.|Cell Prolif. 2021 Dec 24;e13172.|Clin Transl Med. 2023 Mar;13(3):e1229.|Environ Toxicol. 2023 May 20.|Free Radic Biol Med. 2023 Sep 1;208:402-417.|Int J Mol Med. 2023 Apr 7.|Int J Mol Sci. 2023 Sep 5, 24(18), 13812.|Microbiol Spectr. 2023 May 1;e0313822.|Nat Commun. 2023 Jun 30;14(1):3877.|SSRN. 2023 Mar 9.|SSRN. 2023 Nov 15.
References
– [1]Darzynkiewicz Z, et al. Differential staining of DNA and RNA. Curr Protoc Cytom. 2004 Nov;Chapter 7:Unit 7.3.|[2]Mirrett S. Acridine orange stain. Infect Control. 1982 May-Jun;3(3):250-2. |[3]Yektaeian N, et al. Lipophilic tracer Dil and fluorescence labeling of acridine orange used for Leishmania major tracing in the fibroblast cells. Heliyon. 2019 Dec 18;5(12):e03073.
CAS Number
– 494-38-2
Molecular Weight
– 265.35
SMILES
– CN(C)C1=CC=C2C(N=C3C=C(N(C)C)C=CC3=C2)=C1
Clinical Information
– No Development Reported
Research Area
– Others
Solubility
– 10 mM in DMSO
Target
– Parasite
Pathway
– Anti-infection
Product type
– Dye Reagents
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
