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5-HTP(5-Hydroxytryptophan) ELISA Kit
$212 – $301
Products Details
Product Description
– This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-HTP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-HTP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-HTP in the samples is then determined by comparing the OD of the samples to the standard curve.
Web ID
– IM4218KLE
Applications
– Competitive Inhibition ELISA
Research Area
– Enzyme & Kinase,
Features & Benefits
– Competitive Inhibition ELISA with sensitivity of 1.77 ng/mLand detection range of 6.25-400 ng/mL. Kit includes a standard of 400 ng/mL and takes a total assay time of 2h. This kit can be used to assay serum, plasma and other biological fluids.
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.
