– 5′-Azido-5′-deoxythymidine is a nucleoside analog with potential anti-tuberculosis activity as an inhibitor of Mycobacterium tuberculosis monophosphate kinase (TMPKmt), an enzyme essential for DNA replication in Mycobacterium tuberculosis. 5′-Azido-5′-deoxythymidine contains an azide group in its molecular structure, which enhances its ability to inhibit reverse transcriptase, and is commonly used in the study of nucleoside metabolic pathways and related disease mechanisms.