– VU0506013 is a high-affinity positive allosteric modulator (PAM) of the neuropeptide Y4 receptor. This compound demonstrates nanomolar-level affinity and notable selectivity in engineered cell lines and the descending colon mucosa of mice that naturally express Y4R. VU0506013 presents a promising foundation for developing in vivo tools aimed at advancing anti-obesity compound research targeting Y4R.