Tris chloride buffer salt

– Tris chloride buffer salt is a commonly used salt in molecular biology experiments, which is composed of trimethylmethanyl methyl salt (Tris) and potassium fertilizer (NaCl). It has special features such as adjusting pH value and ionic strength, high solubility and low toxicity, and is widely used in DNA/RNA extraction, PCR, protein electrophoresis and other experiments. In DNA/RNA extraction experiments, Tris chloride buffer salt is usually used for DNA/RNA purification, accelerated reaction process and balanced determination of DNA/RNA structure, etc. Can effectively protect DNA/RNA from deoxyribonuclease (DNase) and ribonuclease (RNase) degradation. in PCR experiments. It can be used for the preparation of PCR reaction system. At the same time, Tris chloride buffer can also increase the concentration of the reaction mixture, thereby improving PCR. In protein electrophoresis experiments, Tris chloride buffer salt can be used as a gel flushing solution to maintain the stability of the gel and the pH value of the electrophoresis solution. . In conclusion, Tris chloride buffer salt is a commonly used salt for molecular biology experiments, which has the characteristics of adjusting pH value and ionic strength, high temperature solubility and low toxicity.