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Products Details
Product Description
– AmplifyRP® XRT for ToBRFV is a rapid RNA amplification and detection platform designed for testing peppers and tomatoes for Tomato brown rugose fruit virus. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify ToBRFV RNA and an endogenous RNA control at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( – ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( – ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
Web ID
– XCS 66800
Shipping
– Room temperature
Kit Components
– XRT reaction pellets for ToBRFV
Pre-filled 100 µL PD1 Pellet diluent tubes
GEB-filled mesh extraction bags
User Guide
Pre-filled 100 µL PD1 Pellet diluent tubes
GEB-filled mesh extraction bags
User Guide
Features & Benefits
– Test Format: AmplifyRP® XRT
Target Label: FAM-labeled target probe
Internal Control: ROX-labeled control probe (endogenous)
Limit of detection: Approximately 15 fg/µL of RNA transcript
Limit of detection: 1:10,000,000 dilution of infected tissue (pathogen titer unknown)
Target Label: FAM-labeled target probe
Internal Control: ROX-labeled control probe (endogenous)
Limit of detection: Approximately 15 fg/µL of RNA transcript
Limit of detection: 1:10,000,000 dilution of infected tissue (pathogen titer unknown)
Disclaimer: All products are for Research use only unless clearly stated otherwise on the product datasheet. Datasheets provided on the website are drafts for reference purpose only and you are requested to always refer to the hard copy included in the kit for your experimentation. Agdia Products are available for delivery only in Canada.